vAC is hardcoded to RAM address 0x0018-0x0019, LDWI for example loads a 16bit immediate value and stores it into these RAM addresses. In your assembler just setup a symbol, (e.g. vAC), that represents the RAM addresse 0x18 and then you can use it as I showed above.
CALL can use any zero page RAM address, e.g. CALL 0x18, which conceptually is CALL vAC.
What is the difference between the 32k and 64k version?
Only allocation for code.
In 32K code is allocated in first 32kB, in 64K above 32kB. I did my first test on emulator with 64kB and it was easer for me.
Data for graphics is allocated in the same palce.